| 刘艳萍,殷燕,苏玫匀,袁志兵,伍丽,谢和兵.藏药五味姜黄丸HPLC指纹图谱及指标成分含量测定研究[J].,2025,34(8):65-73 |
| 藏药五味姜黄丸HPLC指纹图谱及指标成分含量测定研究 |
| Study on HPLC Fingerprint and Content Determination of Index Components of Zang Medicine Wuwei Jianghuang Pills |
| 投稿时间：2024-08-01 |
| DOI： |
| 中文关键词: 藏药五味姜黄丸 高效液相色谱法 含量测定 指纹图谱 |
| 英文关键词: Wuwei Jianghuang Pills of Zang Medicine High Performance Liquid Chromatography Content Determination Chromatographic Fingerprinting |
| 基金项目:国家自然科学基金资助项目(81470247);西藏自治区科技厅区域科技协同创新专项(QYXTZX-RKZ2022-07)。 |
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| 中文摘要: |
| 目的：建立藏药五味姜黄丸HPLC指纹图谱，并同时测定指标成分没食子酸、木兰花碱、鞣花酸、药根碱、盐酸小檗碱、双去甲氧基姜黄素、去甲氧基姜黄素、姜黄素的含量。方法：以自制的10批五味姜黄丸为研究样品，采用C18色谱柱，柱温30℃,以0.1%磷酸水(A)-乙腈(B)梯度洗脱，流速1.0mL/min,检测波长270nm,建立HPLC指纹图谱，并分析指纹图谱相似度和测定指标成分含量。结果：五味姜黄丸HPLC指纹图谱共标识出26个共有峰，指纹图谱相似度＞0.99;指标成分没食子酸、木兰花碱、鞣花酸、药根碱、盐酸小檗碱、双去甲氧基姜黄素、去甲氧基姜黄素、姜黄素分别在2.670~26.696μg/mL、9.126~91.260μg/mL、1.525~15.245μg/mL、0.812~8.121μg/mL、5.042~50.418μg/mL、4.037~40.366μg/mL、4.026~40.257μg/mL、4.709~47.088μg/mL的范围内线性良好，相关系数r分别为1.0000、1.0000、0.9993、1.0000、1.0000、0.9991、0.9993、0.9995;稳定性、精密度、重复性试验的RSD(n=6)均小于2%;平均回收率分别为93.969%、101.069%、100.961%、100.595%、99.614%、101.668%、99.894%、101.908%,RSD(n=6)分别为0.294%、1.835%、0.191%、0.229%、0.844%、0.861%、1.125%、0.411%。结论：建立的指纹图谱以及多指标含量测定方法准确稳定，重复性好，可为五味姜黄丸的质量控制以及标准建立提供参考。 |
| 英文摘要: |
| Objective The HPLC fingerprints of Wuwei Jianghuang pills of Zang medicine were established , and the contents of the index components gallic acid , magnoflorine , ellagic acid , jatrorrhizine , berberine hydrochloride , bisdemethoxycurcumin , demethoxycurcumin and curcumin were determined at the same time. Methods Ten batches of Wuwei Jianghuang pills made in - house were used as the study samples , and the HPLC fingerprints were established by using a C 18 column at a column temperature of 30 ℃ with a gradient elution of 0. 1% phosphoric acid in water (A) - acetonitrile (B) , at a flow rate of 1 . 0 mL/min , and a detection wavelength of 270 nm , and the fingerprints were analysed for the similarity of the fingerprints and the determination of the content of indicator in- gredients. Results A total of 26 common peaks were identified in the HPLC fingerprint of Wuwei Jianghuang pills , and the similarity of the fingerprints was > 0. 99. The indicator concentrations of gallic acid , magnoflorine , ellagic acid , jatrorrhizine , berberine hydrochlo ride , bisdemethoxycurcumin , demethoxycurcumin and curcumin were in the range of 2. 670 - 26. 696 μg/mL , 9. 126 - 91 . 260 μg/ mL , 1 . 525 - 15. 245 μg/mL , 0. 812 - 8. 121 μg/mL , 5. 042 - 50. 418 μg/mL , 4. 037 - 40. 366 μg/mL , 4. 026 - 40. 257 μg/mL , and 4. 709 - 47. 088 μg/mL , respectively , and the correlation coefficients rwere good with a range of 1 . 0000 , 1 . 0000 , 0. 9993 ,1 . 0000 , 1 . 0000 , 1 . 0000 , 0. 9991 , 0. 9993 , 0. 9995 ; the RSDs of the stability , precision and reproducibility tests were less than 2% ; and the average recoveries were 93 . 969% , 101 . 069% , 100. 961% , 100. 595% , 99. 614% , 101 . 668% , 99. 894% ,101 . 908% , RSD ( n = 6) were 0. 294% , 1 . 835% , 0. 191% , 0. 229% , 0. 844% , 0. 861% , 1 . 125% , 0. 411% . Conclusion The established fingerprint and multi - indicator content determination method is accurate , stable and reproducible , which can provide a reference for the quality control of Wuwei Jianghuang pills and the establishment of standards. |
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