| 王贺伟,尹强,尹海龙,穆丹丹,田芳,李超杰.维药茴芹果药材中异荭草苷的含量测定[J].,2025,34(8):56-58 |
| 维药茴芹果药材中异荭草苷的含量测定 |
| Determination of the Content of Isoanthocyanin in the Uyghur Medicine Pimpinellae Anisi Fructus |
| 投稿时间：2024-07-23 |
| DOI： |
| 中文关键词: 茴芹果 异荭草苷 高效液相色谱 |
| 英文关键词: Pimpinellae Anisi Fructus Isoorientin HPLC |
| 基金项目:新疆地产药材配方颗粒研制及中试技术平台搭建（编号：2022LQ03020);新疆维吾尔自治区“天山英才”-优秀工程师培养计划项目。 |
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| 中文摘要: |
| 目的：建立茴芹果中异荭草苷的含量测定方法。方法：利用HPLC法，以Aglient-ZORBAX-Eclipse-PlusC18柱为色谱柱，使用甲醇-0.1%磷酸为流动相（梯度洗脱），检测波长为347nm,测定茴芹果药材中异荭草苷的含量。结果：异荭草苷浓度在3.625~64.108μg/mL范围时，峰面积与浓度呈良好的线性关系(R2=0.9994),平均加样回收率101.8408%(RSD=3.5%,n=9)。异荭草苷含量为292.1803~491.4936μg/g。结论：建立的含量测定方法，容易操作，可控性良好，适用于茴芹果的质量控制。 |
| 英文摘要: |
| Objective Establish a method for determining the content ofisoorientin in Pimpinellae anisi fructus. Method High - performance liquid chromatography (HPLC) was employed using an Agilent ZORBAX Eclipse Plus C 18 column , with a mobile phase of methanol - 0. 1% phosphoric acid (gradient elution) . Detection was conducted at a wavelength of 347nm to determine the content of isoorientin in Pimpinellae anisi fructus. Results A good linear relationship was observed between the peak area and concentration of isoorientin in the range of 3 . 625 to 64. 108 μg/g (R2 = 0. 9994) . The average sample recovery rate was 101 . 8408% (RSD = 3 . 5% , n = 9) . The content of isoorientin ranged from 292. 1803 to 491 . 4936 μg/g. Conclusion The established determination method is easy to operate with good controllability , suitable for quality control of Pimpinellae anisi fructus. |
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