| 覃丽郦,何颂华,罗轶,谷立勍.LC-MS法测定水田七中根薯酮内酯A和根薯酮内酯B[J].,2025,34(5):65-67 |
| LC-MS法测定水田七中根薯酮内酯A和根薯酮内酯B |
| Analysis of Taccalonolide A and Taccalonolide B in Schizocapsa Plantaginea Rhizoma by Liquid Chromatography - Mass Spectrometry |
| 投稿时间：2024-06-13 |
| DOI： |
| 中文关键词: 水田七；根薯酮内酯A 根薯酮内酯B 液质联用 |
| 英文关键词: Schizocapsa Plantaginea Rhizoma Taccalonolide A Taccalonolide B LC - MS |
| 基金项目:广西药品监督管理局科研计划（桂药监科2019-10);广西常用壮瑶药药材质量标准研究（桂科AB17292069)。 |
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| 中文摘要: |
| 目的：建立水田七中根薯酮内酯A和根薯酮内酯B的液质联用检测(LC-MS)和分析方法。方法：采用Kromasil ProshellC18 柱(2.5μm,2.1mm×100mm)色谱柱，以乙腈-0.1%甲酸溶液为流动相，梯度洗脱，多反应监测模式进行检测分析。结果：根薯酮内酯A和根薯酮内酯B进样量分别在10.1136~505.6800pg和0.9996~99.9600pg范围内线性关系良好，r>0.999,加样回收率分别为95.45%(RSD2.88%),99.12%(RSD2.82%)。结论：所建立的方法快速、准确、灵敏、可靠，能够用于水田七中根薯酮内酯A和根薯酮内酯B的测定，为其质量控制研究提供科学依据。 |
| 英文摘要: |
| Objective To establish the analysis method of taccalonolide A and taccalonolide B in Schizocapsa Plantaginea Rhizoma by liquid chromatography - mass spectrometry (LC - MS) .Methods An Kromasil Proshell C 18 (2.5μm ,2.1mm×100 mm ) col- umn was used with gradient elution of acetonitrile - 0.1% formic acid.The multiple - reaction monitoring mode was used. As a result ,the LC - MS method was established to detect the taccalonolide A and taccalonolideB in Schizocapsa Plantaginea Rhizoma. And the method was further validated and used to test all samples.Results The developed method showed good linearity in the range of 10. 1136 - 505. 6800 pg , 0.9996 - 99.9600 pg with r > 0.999 for taccalonolide A and taccalonolide B ,respectively. The recovery rates were 95. 45% (RSD 2. 88% ),99. 12% (RSD 2.82% ),respectively. Conclusion The established method is accurate , sen- sitive and reliable.It can be applied for analysis of taccalonolide A and taccalonolide B in Schizocapsa Plantaginea Rhizoma. And it can provide sound scientific basis for the study of Schizocapsa plantaginea rhizome quality control. |
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