| 吴倩,侯安国,朱常成,尹子丽.大孔树脂纯化蒙自藜芦总生物碱提取工艺研究[J].,2025,34(1):44-48 |
| 大孔树脂纯化蒙自藜芦总生物碱提取工艺研究 |
| Study on the Purification Technology of Total Alkaloids from the Veratrum mengtzeanum with Macroporous Resin |
| 投稿时间：2024-04-15 |
| DOI： |
| 中文关键词: 蒙自藜芦 总物碱 大孔树脂 纯化工艺 |
| 英文关键词: Veratrum mengtzeanum Total Alkaloids Macroporous Resin Purification Technology |
| 基金项目:云南省科技厅-云南中医药大学应用基础研究联合专项（202101AZ070001-322202101AZ070001-196，202301AZ070001-034）；云南省教育厅科学研究基金项目（2021J0407，2023J1491）；云南省傣医药与彝医药重点实验师室开放课题（202210ZD2208，202210SS2212）。 |
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| 中文摘要: |
| 目的：对蒙自藜芦总生物碱提取工艺进行优化。方法：以蒙自藜芦总生物碱含量及3个成分藜芦碱、藜芦胺、介芬胺为指标，通过静态吸附和动态吸附实验考查上样体积、上样液质量浓度、径高比、上样液PH值、水洗用量、洗脱剂及其用量，优化出最佳纯化工艺条件。结果：选用D101型大孔树脂纯化蒙自藜芦总生物碱提取物，上样体积50mL，径高比1:5，上样液总生物碱质量浓度为0.2mg/mL，上样液pH值为9，水洗用量为4BV，洗脱剂为80%乙醇，洗脱剂用量为8BV，纯化后的蒙自藜芦总生物碱转移率大于60%，含量为62.88%，比纯化前提高5.74倍。结论：D101型大孔树脂纯化蒙自藜芦总生物碱工艺稳定可行，可为后续制剂开发提供参考依据。 |
| 英文摘要: |
| Objective In Veratrum mengtzeanum total alkaloid extraction technology was optimized. Methods Taking the total al-kaloid content and three components veratrine, veratrine, and mesofenamine as indicators, the sample volume, mass concentration ofsample solution, diameter/height ratio, pH value of sample solution, amount of water washing, eluent and its amount of sample solu-tion were examined by static adsorption and dynamic adsorption experiments, and the best purification process conditions were opti-mized. Results D101 macroporous resin was used to purify the total alkaloid extract of Veratrum mengtzeanum. The sample volume was50mL and the diameter/height ratio was 1:5, on the quality of samples, total alkaloids concentration is 0.2 mg/mL, pH value of thesample fluid 9, water dosage of 4 bv, eluent is 80% ethanol, dosage of eluent is 8 bv, purified of Veratrum mengtzeanum transfer ratemore than 60% of total alkaloid content is 62. 88% , and 5. 74 times higher than that of before purification. Conclusion D101 macro-porous resin purification process is stable and feasible, which can provide a reference for the subsequent preparation development. |
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