| 陆瑞娜,严国波,周美春,梁子聪,覃建锋.水族祛痹方对KOA兔超声表现、病理评分及MMP-3、Collagen II表达的影响[J].,2025,34(1):24-28 |
| 水族祛痹方对KOA兔超声表现、病理评分及MMP-3、Collagen II表达的影响 |
| The Effect of Shui People Qubi Formula on the Ultrasonic Performance, the Pathological Evaluation,the Expression Level of MMP – 3 and Collagen II in KOA Rabbits |
| 投稿时间：2024-04-04 |
| DOI： |
| 中文关键词: 水族祛痹方 膝骨关节炎 MMP-3 Collagen Ⅱ |
| 英文关键词: Shui People Qubi Formula Knee Osteoarthritis MMP –3 Collagen II |
| 基金项目:贵州省卫健委课题项目（gzkwj2023-469）；黔南州科技计划项目（黔科合社字202204号）；黔南民族医学高等专科学校科研基金项目（QNYZ202231，QNYZ202338) |
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| 中文摘要: |
| 目的：探讨水族祛痹方对KOA兔超声表现、病理评分及MMP-3、Collagen Ⅱ表达的影响。方法：将30只长耳大白兔随机分为正常组、模型组、水族祛痹方组，每组10只。以木瓜蛋白酶膝关节腔内注射诱导建立兔膝骨关节炎模型，给与水族祛痹方水煎剂治疗，每日2次。造模后第28天比较超声下关节病变及病理评分，免疫组化检测关节软骨MMP-3、Collagen Ⅱ的表达水平。结果：与正常组比较，模型组超声观察Ridit值及病理Mankins评分明显升高（P<0.05），膝关节软骨MMP-3表达水平明显上升（P<0.05），CollagenⅡ表达水平明显下降（P<0.05）。与模型组比较，水族祛痹方组超声观察Ridit值及病理Mankins评分均明显降低（P<0.05），膝关节软骨MMP-3表达水平明显下降（P<0.05），Collagen Ⅱ表达水平明显上升（P<0.05）。结论：水族祛痹方可以减轻MMP-3对软骨基质的降解，减少软骨胶原丢失，从而减轻滑膜增厚和血管增生，延缓关节退变。 |
| 英文摘要: |
| Objective To study the effect of Shui People Qubi Formula on the ultrasonic performance, the pathological evaluation,the expression level of MMP –3 and Collagen II in KOA rabbits. Methods 30 white rabbits were randomly divided into normal group,model group, and Shui People Qubi Formula treatment group, 10 rabbits in each group. Knee osteoarthritis model was established byintra – articular injection of papain. The rabbits were treated twice a day. 28 days after treatment, the joint characteristic of each groupwas evaluated by ultrasound and pathological detection separately, and MMP –3 and Collagen II expression level in articular cartilagewere detected by immunohistochemistry. Results Compared with the normal group, the Ridit value and pathological Mankins score ofthe model group rabbits significantly increased (P <0.05), MMP –3 expression in knee articular cartilage significantly increased (P<0. 05), and Collagen II expression significantly decreased (P <0. 05). Compared with the model group, the R value and pathologi-cal Mankins score of rabbits in the Shui People Qubi Formula treatment group significantly decreased (P <0. 05), MMP –3 expressionin knee articular cartilage significantly decreased (P <0. 05), and Collagen II expression significantly increased (P <0.05). Conclusion Shui People Qubi Formula can reduce the degeneration of cartilage matrix causing by MMP – 3, it also can alleviate synovial proliferation and reduce knee joint angiogenesis, which is to delay joint degeneration. |
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