| 徐清,陈荣林,吴倩媛,王英豪,林建英.基于响应面设计优化姬松茸多糖的提取工艺[J].,2024,33(14):48-55 |
| 基于响应面设计优化姬松茸多糖的提取工艺 |
| Optimization of Agaricus Blazei Polysaccharide Extraction Process Based on Response Surface Design |
| 投稿时间：2023-10-27 |
| DOI： |
| 中文关键词: 姬松茸 超声波 酶提法 响应面设计 多糖提取 |
| 英文关键词: Agaricus Blazei Ultrasound Enzymatic Extraction Box -Benhnken |
| 基金项目: |
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| 摘要点击次数: 1965 |
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| 中文摘要: |
| 目的:运用响应面设计优化姬松茸多糖的提取工艺。方法:以超声波提取与复合酶酶解法提取姬松茸中的多糖成分，以复合酶添加量、pH值、超声温度、提取时间为考察因素，在单因素实验的基础上，运用响应面设计优化姬松茸多糖的提取工艺。结果:最佳提取工艺条件为复合酶添加量0.03g、pH6.5、超声温度70 ℃、提取时间 20 min，此条件下姬松茸多糖的得率为10.67%，与预测值11.06%接近。结论:优选出的提取工艺稳定可行，有助于姬松茸的合理运用，为姬松茸多糖有效提取和科学开发提供了参考。 |
| 英文摘要: |
| Objectives The aim of this project is to optimise the extraction process of Agaricus blazei polysaccharides by using Box- Benhnken. Methods The polysaccharide components in Agaricus blazei were extracted by ultrasonic wave extraction and complex en-zyme hydrolysis, and the complex enzyme addition, pH value, ultrasonic temperature and extraction time were investigated, Box -Benhnken was used to optimize the extraction process of Agaricus blazei polysaccharide. Results The optimum extraction process condi-tions were 0. 03g of complex enzyme addition, pH 6. 5, ultrasonic temperature of 70C and extraction time of 20min. The yield ofAgaricus blazei polysaccharide under these conditions was 10. 67%, which was close to the predicted value of 11. 06%. ConclusionThe extraction process is stable and feasible, promoting the rational use of Agaricus blazei, and providing a reference for the effectiveextraction and scientific development of Agaricus blazei polysaccharides. |
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