| 张利军,龙小妹,李蓉,夏宗霄,郭爽,古建兴,刘海鹏,范源.HPLC法同时测定余甘子果核中6种鞣质类成分的含量[J].,2024,33(1):62-66 |
| HPLC法同时测定余甘子果核中6种鞣质类成分的含量 |
| HPLC Method was Used to Determine the Content of Six Tannins in the Kernel of Phyllanthus emellia |
| 投稿时间：2023-04-03 |
| DOI： |
| 中文关键词: 余甘子 果核 鞣质类成分 HPLC |
| 英文关键词: Phyllanthus Emellia Kernel Tannin Components HPLC |
| 基金项目:国家自然科学基金地区基金项目（81760856）；云南省科技厅科技计划项目-应用基础研究计划（202101AZ010001-015）；云南省“高层次人才培养支持计划”（名医专项）；云南中医药大学第二附属医院中医特色专科内分泌科建设项目。 |
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| 中文摘要: |
| 目的：建立同时测定余甘子果核中没食子酸（Gallic acid)、柯里拉京（Corilagin)、没食子基儿茶素没食子酸酯((-)-Gallocatechin gallate,GCG)、诃黎勒酸(Chebulagic acid)、1,2,3,4,6-0-五没食子酰葡萄糖(1,2,3,4,6-Pentagalloylglucose,PGG)、鞣花酸(Ellagic acid)6种鞣质类成分的HPLC方法。方法：采用Agilent ZorbaxCs色谱柱（250mm×4.6mm,5μm）,流动相为甲醇（A）-0.1%磷酸（B）,梯度洗脱：流速：1.0mL·min-1；检测波长：270nm；柱温：25℃。结果：余甘子果核中6个成分分离度良好，没食子酸、柯里拉京、GCG、诃黎勒酸、PGG、鞣花酸的线性范围分别在0.0836~0.5021 μg、0.0652~0.3912μg、0.0256~0.1536μg、0.0710~0.4260μg、0.0916~0.5496μg、0.2784~1.6704 μg；线性方程分别为Y=2753.9X+8.6667、Y=1013.1X+107.69、Y=4517.3X-24.433、Y=3087.8X+29.947、Y=1097.5X-20.267、Y=7588.6X-364.58范围内与峰面积呈良好的线性关系；精密度、重复性良好，RSD均小于5%；在室温条件下12h内稳定；平均加样回收率在97.05%~102.30%之间（RSD为1.10%~1.90%）。结论：该方法简便、准确，重复性好，可用于余甘子果核中没食子酸、柯里拉京、GCG、诃黎勒酸、PGG、鞣花酸6种成分的同时测定。为余甘子果核的质量研究提供一定的理论依据。 |
| 英文摘要: |
| Objective To establish the simultaneous determination of Gallic acid, Corilagin, Gallocatechin gallate ( ( – ) – Gal-locatechin gallate, HPLC method for six tannins including GCG, Chebulagicacid, 1, 2, 3, 4, 6 – 0 – pentagalloylglucose (1, 2,3, 4, 6 – pentagalloylglucose (PGG) and Ellagic acid. Methods The chromatography was performed on Agilent Zorbax C1g column(250 mm ×4. 6 mm, 5 um) with mobile phase consisted of methanol (A) –0. 1% phosphoric acid (B) and gradient elution at theflow rate of 1. 0 mL · min-1. Detection wavelength: 270 nm; Column temperature: 25 ℃. Results The separation degree of 6 compo-nents in the seed of Emellia mongolica was good. The linear ranges of gallic acid, Corritin, GCG, harillic acid, PGG and ellagic acidwere 0. 0836 ~ 0. 5021 μg, 0. 0652 ~ 0. 3912 μg, 0. 0256 ~ 0. 1536 μg, 0. 0710 ~ 0. 4260 μg, 0. 0916 ~ 0. 5496 μg, 0. 2784 ~1. 6704 μg, respectively. The linear equations are Y = 2753. 9X + 8. 6667, Y = 1013. 1X + 107.69, Y = 4517. 3X - 24. 433, Y =3087. 8X + 29. 947, Y = 1097. 5X - 20. 267, Y = 7588. 6X -364. 58 in the range of good line with the peak area Sexual relations; Theprecision and repeatability were good with RSDS less than 5%. Stable within 12 h at room temperature; The average recoveries were97. 05% ~ 102. 30% (RSD 1. 10% ~ 1. 90% ) . Conclusion The method is simple, accurate and reproducible, and can be used for simultaneous determination of gallic acid, corritin, GCG, Harillic acid, PGG and ellagic acid in the fruit core of Emellia japonica. Toprovide a certain theoretical basis for the quality research of the fruit kernel of Phyllanthus emblica. |
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