文章摘要
吴津丽,高晗,曾永祯,吴清雨,陈博涛,李军山,王亚涛,董博宇,张睿智,孙福仁.HPLC法同时测定美洲大蠊标准汤剂中6种成分含量[J].,2023,32(1):32-36,64
HPLC法同时测定美洲大蠊标准汤剂中6种成分含量
Simultaneous Determination of Six Constituents in Standard Decotion of Periplaneta americana by HPLC
投稿时间：2022-05-06  
DOI：
中文关键词: 美洲大蠊  尿嘧啶  次黄嘌呤  尿苷  肌苷  鸟苷  原儿茶酸
英文关键词: Periplaneta americana  Uracil  Hypoxanthine  Uridine  Inosine  Guanosine  Protocatechuic Acid
基金项目:基于标准汤剂的云南省特色中药配方颗粒生产工艺、质量体系及等效性研究(项目编号：202102AA310027)。
作者单位
吴津丽 神威药业集团有限公司，河北 石家庄 051430 
高晗 神威药业集团有限公司，河北 石家庄 051431 
曾永祯 神威药业集团有限公司，河北 石家庄 051432 
吴清雨 神威药业集团有限公司，河北 石家庄 051433 
陈博涛 神威药业集团有限公司，河北 石家庄 051434 
李军山 1.神威药业集团有限公司，河北 石家庄 051430；2.楚雄神威施普瑞药物研究有限公司，云南 楚雄 675000；3.云南省中药配方颗粒工程研究中心，云南 楚雄 675000 
王亚涛 神威药业集团有限公司，河北 石家庄 051430 
董博宇 神威药业集团有限公司，河北 石家庄 051431 
张睿智 神威药业集团有限公司，河北 石家庄 051432 
孙福仁 神威药业集团有限公司，河北 石家庄 051433 
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中文摘要:
      目的：建立HPLC法同时测定美洲大蠊标准汤剂中尿嘧啶、次黄嘌呤、尿苷、肌苷、鸟苷、原儿茶酸的含有量。方法：采用WATERS Symmetry Shield C1g色谱柱（250mm×4.6mm,5um）；甲醇-0.1%磷酸水作为流动相，梯度洗脱；体积流量0.6mL/min；检测波长254nm；柱温20℃。结果：测定显示尿嘧啶、次黄嘌呤、尿苷、肌苷、鸟苷、原儿茶酸分别在0.041~0.341 μg(r=0.9994)、0.097~0.810μg(r=0.9994)、0.020~0.168 μg(r=0.9999)、0.277～2.308 μg(r=0.9999）、0.016~0.135μg（r=0.9997）、0.131~1.096μg（r=0.9998）范围内线性关系良好，平均加样回收率分别为99.93% (RSD =1.18%)、 98.03% (RSD =1.05%)、 97.17% (RSD =1.29%)、 100.19% (RSD =1.91%)、98.94% (RSD=1.11%）、100.70%（RSD=1.05%）。十批美洲大蠊标准汤剂各成分含量范围分别为：尿嘧啶0.14~0.21mg/g、次黄嘌呤0.22~0.36mg/g、尿苷0.06~0.08mg/g、肌苷2.17~3.15mg/g、鸟苷0.03~0.07mg/g、原儿茶酸0.35~0.56mg/g，处于均值的±30%范围内。结论：美洲大蠊标准汤剂含量测定方法准确、可靠，可用于美洲大蠊配方颗粒的质量参考。
英文摘要:
      Objective To establish the HPLC method for simultaneously determining the contents of uracil, hypoxanthine, uri-dine, inosine, guanosine, protocatechuic acid in standard decoction of Periplaneta americana. Methods The analysis was performedon WATERS Symmetry Shield Cg column (250 mm ×4.6 mm, 5 um) . The chromatograhic separation was gradient by methanal –0. 1% phosphoric acid; The flow rate was 0. 6mL/min. The column temperature was 20 T and the detection wavelength was set at254 nm. Results The results showed that Uracil, hypoxanthine, uridine, inosine, guanosine, protocatechuic acid showed good line-ar relationships within the ranges of 0.041 –0. 341 ug (r =0. 9994), 0.097 – 0. 810 ug (r =0. 9994), 0.020 – 0. 168 μg (r =0. 9999), 0. 277 –2.308 μg (r =0. 9999), 0.016 –0. 135 μg (r =0. 9997), 0. 131 - 1.096 μg (r =0. 9998) and their averagerecoveries were 99. 93% (RSD = 1. 18% )、 98. 03% (RSD = 1. 05%)、 97. 17% (RSD = 1. 29% )、 100. 19% (RSD =1. 91%)、 98. 94% (RSD =1. 11%)、 100. 70% (RSD =1. 05%), respectively. The content ranges of each component in ten bat-ches of Standard Decotion of Periplaneta americana were as follows: 0. 14 –0. 21 mg/g of uracil, 0. 22 –0.36 mg/g of hypoxanthine,0. 06 –0. 08 mg/g of uridine, 2. 17 –3. 15 mg/g of inosine, 0. 03 – 0. 07 mg/g of guanosine, 0. 35 – 0. 56 mg/g of protocatechuicacid. The content ranges were in the range of the mean ±30%. Conclusion The method is simple and accurate, which can be usedfor the quality control of Periplaneta americana dispensing granules.
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